Distribution of caprine arthritis encephalitis virus provirus, RNA, and antigen in the reproductive tract of one naturally and seven experimentally infected bucks.

Autor: Turchetti AP; Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, 31.270-901, Belo Horizonte, Brazil., Paniago JJ, da Costa LF, da Cruz JC, Braz GF, Gouveia AM, Paixão TA, Santos RL, Heinemann MB
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2013 Nov; Vol. 80 (8), pp. 933-9. Date of Electronic Publication: 2013 Aug 20.
DOI: 10.1016/j.theriogenology.2013.07.021
Abstrakt: Caprine arthritis encephalitis is a worldwide, multisystemic disease caused by a small ruminant lentivirus. Although the main route of transmission is oral, detection of proviral DNA of the caprine arthritis encephalitis virus (CAEV) in caprine semen has been previously described. However, the presence of viral antigens in the male reproductive tract has apparently never been reported. The objective was to study lesions in the buck reproductive system and to detect, in these tissues, the presence of proviral DNA, viral RNA and CAEV antigens. Tissues from eight CAEV-infected bucks (one naturally and seven experimentally infected) were analyzed by histopathology, nested polymerase chain reaction, reverse transcriptase-polymerase chain reaction, and immunohistochemistry. Interstitial pneumonia, synovitis, and lesions in the male reproductive tract were detected in some of the bucks. Proviral DNA was detected in the lungs and joints as well as in the reproductive systems of all animals, whereas viral RNA was detected only in the genital tract of the naturally infected buck. Viral antigens were immunostained in most of the organs of the male reproductive tract. This report was apparently the first to clearly demonstrate CAEV antigen expression in the male reproductive tract, which indicates the possibility of venereal transmission of CAEV.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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