| Autor: |
VanWagner M; Department of Biomedical Engineering, College of Engineering, Michigan Technological University , 1400 Townsend Drive, Houghton, Michigan 49931, United States., Rhadigan J, Lancina M, Lebovsky A, Romanowicz G, Holmes H, Brunette MA, Snyder KL, Bostwick M, Lee BP, Frost MC, Rajachar RM |
| Jazyk: |
angličtina |
| Zdroj: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2013 Sep 11; Vol. 5 (17), pp. 8430-9. Date of Electronic Publication: 2013 Aug 21. |
| DOI: |
10.1021/am4017945 |
| Abstrakt: |
An S-nitroso-N-acetylpenicillamine (SNAP) derivatization approach was used to modify existing free primary amines found in fibrin (a natural protein-based biomaterial) to generate a controlled nitric oxide (NO) releasing scaffold material. The duration of the derivatization reaction affects the NO release kinetics, the induction of controlled NO-release, hydrophobicity, swelling behavior, elastic moduli, rheometric character, and degradation behavior. These properties were quantified to determine changes in fibrin hydrogels following covalent attachment of SNAP. NO-releasing materials exhibited minimal cytotoxicity when cultured with fibroblasts or osteoblasts. Cells maintained viability and proliferative character on derivatized materials as demonstrated by Live/Dead cell staining and counting. In addition, SNAP-derivatized hydrogels exhibited an antimicrobial character indicative of NO-releasing materials. SNAP derivatization of natural polymeric biomaterials containing free primary amines offers a means to generate inducible NO-releasing biomaterials for use as an antimicrobial and regenerative support for tissue engineering. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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