| Autor: |
Shinde AA; Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431 004, M.S., India., Shaikh FK, Padul MV, Kachole MS |
| Jazyk: |
angličtina |
| Zdroj: |
Saudi journal of biological sciences [Saudi J Biol Sci] 2012 Jul; Vol. 19 (3), pp. 317-23. Date of Electronic Publication: 2012 Mar 19. |
| DOI: |
10.1016/j.sjbs.2012.03.001 |
| Abstrakt: |
Exploring bacterial communities with proteolytic activity from the gut of the Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) insect pests was the purpose of this study. As initial efforts to achieve this goal here we report the isolation of new Bacillus subtillis RTSBA6 6.00 strain from the gut of H. armigera and demonstrated as proteases producer. Zymographic analysis revealed 12 proteolytic bands with apparent molecular weights ranging from 20 to 185 kDa. Although some activity was detected at acidic pH, the major activity was observed at slight alkaline pH (7.8). The optimum temperature was found to be 35 °C with complete loss of activity at 70 °C. All proteases were completely inactivated by PMSF (phenylmethylsulfonyl fluoride) and TPCK (N-tosyl-l-phenylalanine chloromethyl ketone), suggesting that proteases secreted by B. subtillis RTSBA6 6.00 belong to serine proteases class with chymotrypsin-like activity. The occurrence of protease producing bacterial community in the gut of the H. armigera advocates its probable assistance to insect in proteinaceous food digestion and adaptation to protease inhibitors of host plants. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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