Inhibitory effects of cultured Dendrobium tosaense on atopic dermatitis murine model.

Autor: Wu CT; Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan., Huang KS; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan., Yang CH; Department of Biological Science and Technology, I-Shou University, Kaohsiung 84001, Taiwan., Chen YC; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan., Liao JW; Graduate Institute of Veterinary Pathobiology, National Chung-Hsing University, Taichung 40227, Taiwan., Kuo CL; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan., Chen CL; Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan., Lo SF; Department of Agronomy, Chiayi Agricultural Experiment Station, TARI, Chiayi 60044, Taiwan., Hsieh CC; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan; Department of Animal Science and Biotechnology, Tunghai University, Taichung 40704, Taiwan. Electronic address: cchsieh@thu.edu.tw., Tsay HS; Department of Agronomy, National Chung-Hsing University, Taichung 40227, Taiwan; Department and Graduate Institute of Applied Chemistry, Chaoyang University of Technology, Wufeng 41349, Taiwan. Electronic address: hstsay@cyut.edu.tw.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2014 Mar 25; Vol. 463 (2), pp. 193-200. Date of Electronic Publication: 2013 Aug 16.
DOI: 10.1016/j.ijpharm.2013.08.015
Abstrakt: Dendrobium tosaense is one of the most valuable Chinese medicines and well developed health food. Atopic dermatitis (AD) is a chronic skin disease that occurs mainly in childhood. The pathogenesis of atopic dermatitis had been studied in BALB/c mice modeling by skin-inoculated ovalbumin (OVA) with 2,4,6-trinitro-1-chrolobenzene (TNCB). These mice exhibit features of chronic dermatitis, including skin rash, mast cells infiltration, and elevated serum anti-OVA specific IgE and cytokines modulation. In this study, a standardized ethyl acetate extract of D. tosaense (DtE) was used to protect these mice from the OVA/TNCB-induced skin lesions of atopic dermatitis. The results indicated an increased population of natural T regulatory cell was accompanied by immunosuppression in cytokine profiles and anti-OVA IgE level to significantly reduce Th2 polarization. Finally, toluidine blue staining indicated mast cell infiltration and degranulation was reduced in skin lesion. Our results were shed light on the usage of D. tosaense in AD.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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