A sepiolite modified conducting polymer based biosensor.

Autor: Soylemez S; Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey. Electronic address: saniyesoylemez@gmail.com., Kanik FE; Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey. Electronic address: fulyaekiz@gmail.com., Tarkuc S; Chemical Engineering, Delft University of Technology, The Netherlands. Electronic address: s.tarkuc@tudelft.nl., Udum YA; Institute of Science and Technology, Department of Advanced Technologies, Gazi University, 06000, Turkey. Electronic address: y.udum@gazi.edu.tr., Toppare L; Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey; Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Polymer Science and Technology, Middle East Technical University, Ankara 06800, Turkey; The Center for Solar Energy Research and Application (GUNAM), Middle East Technical University, Ankara 06800, Turkey. Electronic address: toppare@metu.edu.tr.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2013 Nov 01; Vol. 111, pp. 549-55. Date of Electronic Publication: 2013 Jul 12.
DOI: 10.1016/j.colsurfb.2013.07.013
Abstrakt: A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced for a successful immobilization of the cholesterol oxidase. Immobilized enzyme kinetic parameters (KM(app), Imax) were evaluated by Michaelis-Menten kinetics and calculated as 0.031 mM and 6.06 μA, respectively. LOD and sensitivity were estimated as 0.36 μM and 1.64 mA/mMcm(2). Characterization of designed biosensor was done to examine the effect of various factors such as enzyme amount, optimum pH and shelf-life. A novel accurate and inexpensive cholesterol biosensor was developed for the determination of total cholesterol in food samples.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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