| Autor: |
Perez-Pinera P; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA., Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2013 Oct; Vol. 10 (10), pp. 973-6. Date of Electronic Publication: 2013 Jul 25. |
| DOI: |
10.1038/nmeth.2600 |
| Abstrakt: |
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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