| Autor: |
Serrano Mde L; Instituto Superior de Investigaciones Biológicas (CONICET),Universidad Nacional de Tucumán,Departamento de Biología del Desarrollo,San Miguel de Tucumán,Argentina., Luque ME; Instituto Superior de Investigaciones Biológicas (CONICET),Universidad Nacional de Tucumán,Departamento de Biología del Desarrollo,San Miguel de Tucumán,Argentina., Sánchez SS; Instituto Superior de Investigaciones Biológicas (CONICET),Universidad Nacional de Tucumán,Departamento de Biología del Desarrollo,Chacabuco 461,T4000ILI San Miguel de Tucumán,Argentina. |
| Jazyk: |
angličtina |
| Zdroj: |
Zygote (Cambridge, England) [Zygote] 2015 Feb; Vol. 23 (1), pp. 99-110. Date of Electronic Publication: 2013 Jul 26. |
| DOI: |
10.1017/S0967199413000324 |
| Abstrakt: |
The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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