| Autor: |
Fernández ME; Facultad de Ciencias Veterinarias, Instituto de Genética Veterinaria, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata, La Plata, Argentina ., Goszczynski DE, Lirón JP, Villegas-Castagnasso EE, Carino MH, Ripoli MV, Rogberg-Muñoz A, Posik DM, Peral-García P, Giovambattista G |
| Jazyk: |
angličtina |
| Zdroj: |
Genetics and molecular biology [Genet Mol Biol] 2013 Jul; Vol. 36 (2), pp. 185-91. Date of Electronic Publication: 2013 Jun 22. |
| DOI: |
10.1590/S1415-47572013000200008 |
| Abstrakt: |
During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching probability (MP) yielded values greater than 0.9998 and 4.32E(-42), respectively. Generally 2-3 SNPs per STR were needed to obtain an equivalent Q value. The MP showed that 24 SNPs were equivalent to the ISAG (International Society for Animal Genetics) minimal recommended set of 12 STRs (MP ∼ 10(-11)). These results provide valuable genetic data that support the consensus SNP panel for bovine genetic identification developed by the Parentage Recording Working Group of ICAR (International Committee for Animal Recording). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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