| Autor: |
Mulvana DE; Department of Metabolism and Pharmacokinetics, Bristol-Myers Company, Syracuse, NY 13221., Kaul S, Dandekar KA, Pittman KA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1990 May 18; Vol. 527 (2), pp. 343-50. |
| DOI: |
10.1016/s0378-4347(00)82117-9 |
| Abstrakt: |
Belfosdil and the internal standard were extracted from human plasma by a double liquid-liquid extraction. After a concentration step, gas chromatographic analysis of the sample was performed using a capillary fused-silica column and a nitrogen-phosphorus detector. The limit of detection of belfosdil was 0.025 ng/ml and the standard curve was linear over the range 0.05-100 ng/ml. The intra-assay and inter-assay precisions were within 7% (relative standard deviation) and the intra-assay and inter-assay accuracy values deviated by less than 5%. The extractability of belfosdil was 79%. The assay method was successfully used for the analysis of plasma samples from clinical studies with dose ranges of 5-100 mg of belfosdil. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
