| Autor: |
Isaguirre AC; Instituto de Química de San Luis, Facultad de Química, Bioquímica y Farmacia, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Luis, Bloque III, Avda. Ejército de los Andes 950, San Luis, CP 5700, Argentina., Olsina RA, Martinez LD, Lapierre AV, Cerutti S |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2013 Sep; Vol. 405 (23), pp. 7397-404. Date of Electronic Publication: 2013 Jul 23. |
| DOI: |
10.1007/s00216-013-7193-6 |
| Abstrakt: |
Monitoring carnitine and acetylcarnitine levels in biological fluids is a powerful tool for diagnostic studies. Research has recently shown that the analysis of carnitine and related compounds in clinical samples can be accomplished by different analytical approaches. Because of the polar and ionic nature of the analytes and matrix complexity, accurate quantitation is a highly challenging task. Thus, sample processing factors, preparation/cleanup procedures, and chromatographic/ionization/detection parameters were evaluated. On the basis of the results obtained, a rapid, selective, sensitive method based on hydrophilic interaction liquid chromatography-tandem mass spectrometry for the analysis of carnitine and acetylcarnitine in serum and urine samples is proposed. The matrix effect was assessed. The proposed approach was validated, the limits of detection were in the nanomolar range, and carnitine and acetylcarnitine levels were found within the micromolar range in both types of sample. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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