Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections.

Autor: Pulcrano G; Department of Molecular Medicine and Medical Biotechnology, Medicine School, University of Naples Federico II, via Pansini, 80131, Naples, Italy. giovannapulcrano@libero.it, Iula DV, Vollaro A, Tucci A, Cerullo M, Esposito M, Rossano F, Catania MR
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2013 Sep; Vol. 94 (3), pp. 262-6. Date of Electronic Publication: 2013 Jul 09.
DOI: 10.1016/j.mimet.2013.07.001
Abstrakt: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) fingerprinting has recently become an effective instrument for rapid microbiological diagnostics and in particular for identification of micro-organisms directly in a positive blood culture. The aim of the study was to evaluate a collection of 82 stored yeast isolates from bloodstream infection, by MALDI-TOF MS; 21 isolates were identified also directly from positive blood cultures and in the presence of other co-infecting micro-organisms. Of the 82 isolates grown on plates, 64 (76%) were correctly identified by the Vitek II system and 82 (100%) by MALDI-TOF MS; when the two methods gave different results, the isolate was identified by PCR. MALDI-TOF MS was unreliable in identifying two isolates (Candida glabrata and Candida parapsilosis) directly from blood culture; however, direct analysis from positive blood culture samples was fast and effective for the identification of yeast, which is of great importance for early and adequate treatment.
(© 2013. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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