Afrormosin, an Isoflavonoid from Amburana cearensis A. C. Smith, Modulates the Inflammatory Response of Stimulated Human Neutrophils.

Autor: de Araújo Lopes A; Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil., Magalhães TR; Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil., de Andrade Uchôa DE; Department of Organic and Inorganic Chemistry, Federal University of Ceará (UFC), Fortaleza, Brazil., Silveira ER; Department of Organic and Inorganic Chemistry, Federal University of Ceará (UFC), Fortaleza, Brazil., Azzolini AE; Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil., Kabeya LM; Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil., Lucisano-Valim YM; Department of Physics and Chemistry, University of São Paulo (USP), São Paulo, Brazil., Vasconcelos SM; Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Brazil., de Barros Viana GS; Department of Physiology and Pharmacology, Federal University of Ceará (UFC), Fortaleza, Brazil., Leal LK; Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Faculty of Pharmacy, Odontology and Nursing, Federal University of Ceará (UFC), Fortaleza, Brazil.
Jazyk: angličtina
Zdroj: Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2013 Dec; Vol. 113 (6), pp. 363-9. Date of Electronic Publication: 2013 Jul 22.
DOI: 10.1111/bcpt.12106
Abstrakt: Isoflavones are phytoestrogens known by their anti-inflammatory, antioxidant and immunomodulatory properties. Presently, there is no information on whether afrormosin, an isoflavone from Amburana cearensis A.C. Smith (Fabaceae), has some effect on the inflammatory response from stimulated human neutrophils. Thus, the aim of this study was to evaluate the anti-inflammatory and antioxidant potentials of afrormosin on human neutrophils. Neutrophils (2.5 × 10(6) cells/mL) were incubated with afrormosin (3.35-335.2 μM) prepared from a product isolated from Amburana cearensis A.C. Smith with a 78.5% degree of purity and stimulated by the addition of cytochalasin B and N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate-13-acetate (PMA). Afrormosin inhibited the neutrophil degranulation induced by fMLP (10.47-335.2 μM) or PMA (0.33-167.6 μM), myeloperoxidase activity (3.3-335.2 μM), TNF-α secretion (16.7-335.2 μM) and the reactive oxygen species (ROS) generation (16.7-335.2 μM). On the other hand, afrormosin did not show any effect either on elastase or as a free radical scavenger. These data suggest that afrormosin modulates intermediary steps of the neutrophil ROS generation process. In addition, the modulatory effect of afrormosin on human neutrophil degranulation seems to be directed towards PMA-induced activation, indicating a potent inhibition of the protein kinase C activity. This study provided evidence, for the first time, to support the anti-inflammatory and antioxidant activities of afrormosin, creating novel insights into the pharmacological actions of this natural isoflavone.
(© 2013 Nordic Pharmacological Society. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: