[The application of external and internal control objects in case of using of polymerase chain reaction and reverse transcription of polymerase chain reaction].

Autor: Sizikova TE, Mel'nikova EV, Manoshkin AV, Petrov AA, Mel'nikov DG, Pantiukhov VB, Lebedev VN, Borisevich SV
Jazyk: ruština
Zdroj: Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2013 Mar (3), pp. 41-4.
Abstrakt: The external and internal control samples are used in case of polymerase chain reaction application to ensure validity of results. The external control samples are used to establish operability of reagents included into diagnostic kit. The internal control samples make it possible to check not only all the stages of reaction but also the process of amplification in each individual test tube with reaction mixture.
Databáze: MEDLINE