| Autor: |
Furukawa K; Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030., Sahasrabuddhe CG |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1990 Jul 20; Vol. 131 (1), pp. 105-12. |
| DOI: |
10.1016/0022-1759(90)90239-r |
| Abstrakt: |
A novel method for activating fresh human B cell, using liposomes decorated with Fab' fragments of anti-human IgM antibodies (immunoloposomes), is described. In our immunoliposome preparation, more than 80% of Fab' were efficiently bound to liposomes and over 95% of the vesicles were uniformly coated with Fab'. Immunoliposomes could activate fresh B cells from peripheral blood of normal donors at least 100-fold better than soluble F(ab')2 fragments, as detected by [3H]uridine uptake. Such activation was also reflected in the enlarged cell size and highly increased [3H]thymidine uptake by B cells when human B cell growth factor (BCGF) was added. Density of Fab' on the liposomes surface did not affect the efficacy of immunoliposomes. The activation of B cells by immunoliposomes was short lived (less than 3 days), due perhaps to rapid internalization of the vesicles by B cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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