| Autor: |
Tuttolomondo MV; IQUIMEFA-CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 Piso 3º, 1113, Buenos Aires, Argentina., Villanueva ME, Alvarez GS, Desimone MF, Díaz LE |
| Jazyk: |
angličtina |
| Zdroj: |
Biotechnology letters [Biotechnol Lett] 2013 Oct; Vol. 35 (10), pp. 1571-7. Date of Electronic Publication: 2013 Jun 26. |
| DOI: |
10.1007/s10529-013-1259-6 |
| Abstrakt: |
The synthesis of monodispersed magnetic silica nanoparticles (MSN) is described using a water-in-oil reverse microemulsion system that does not require the use of co-surfactants. Sodium silicate, Tween 20 as a neutral surfactant and 1-butanol as the organic phase were used. There are several advantages of the proposed method including a saturation magnetization value of 10 emu/g for the particles obtained, uniformity of size and that they are easily functionalized to bind urease covalently. Moreover, the intra-day, inter-day and long-term stability results confirm that the procedure was successful and the enzyme-linked MSNs were stable over repeated uses and storage retaining more than 75% activity after 4 months. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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