| Autor: |
Sheath KL; Diagnostic Genetics, LabPlus, Auckland City Hospital, Auckland 1148, New Zealand., Duffy L, Asquith P, Love DR, George AM |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular medicine reports [Mol Med Rep] 2013 Aug; Vol. 8 (2), pp. 650-4. Date of Electronic Publication: 2013 Jun 13. |
| DOI: |
10.3892/mmr.2013.1519 |
| Abstrakt: |
The aim of the present study was to evaluate the use of KaryoLite™ bacterial artificial chromosomes (BACs)‑on‑Beads™ (BoBs) technology for the rapid screening of products of conception (POC). Validation and prospective studies were carried out on 85 and 95 patient samples, respectively. Validation studies had previously been analyzed using routine culture and G-banded karyotyping. BoBs resulted in an abnormality detection frequency of 27%, with a failure rate of <3%. The time required for processing was significantly lower compared with that of tissue culture. In conclusion, BoBs technology decreased the failure rate, while increasing the analytical sensitivity compared with G-banded karyotype analysis alone. Additionally, significant cost savings may be achieved with regard to the time of processing and analysis of specimens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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