Hepatoprotective activity of puerarin against carbon tetrachloride-induced injuries in rats: a randomized controlled trial.

Autor: Xia DZ; College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China. xdz_zjtcm@hotmail.com, Zhang PH, Fu Y, Yu WF, Ju MT
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2013 Sep; Vol. 59, pp. 90-5. Date of Electronic Publication: 2013 Jun 10.
DOI: 10.1016/j.fct.2013.05.055
Abstrakt: The protective effects of puerarin on liver damage were evaluated by carbon tetrachloride (CCl₄)-induced hepatotoxicity in rats. Male rats were orally treated with puerarin daily, and received CCl₄ intraperitoneally twice a week for 4 weeks. Our results showed that puerarin at doses of 50, 100, and 200 mg/kg b. w. significantly reduced the elevated activities of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase at least 15%, 17%, 14% and 18%, respectively. In addition, puerarin at different doses significantly decreased (p<0.05) the level of hepatic thiobarbituric acid reactive substances compared to the CCl₄-treated group. Furthermore, the treatment of puerarin was also found to significantly increase the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and glutathione content at least 40%, 12%, 25%, 52%, 17% and 44% in the liver of CCl₄-treated rats, respectively. Liver histopathology also showed that puerarin reduced the incidence of liver lesions induced by CCl₄. The results suggest that puerarin exhibits potent hepatoprotective effects on CCl₄-induced liver damages in rats, and that the hepatoprotective effects of puerarin may be due to both the inhibition of lipid peroxidation and to increase of antioxidant enzymes activity.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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