Cell culture and electron microscopy for identifying viruses in diseases of unknown cause.

Autor: Goldsmith CS; Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G32, Atlanta, GA 30329, USA. cgoldsmith@cdc.gov, Ksiazek TG, Rollin PE, Comer JA, Nicholson WL, Peret TC, Erdman DD, Bellini WJ, Harcourt BH, Rota PA, Bhatnagar J, Bowen MD, Erickson BR, McMullan LK, Nichol ST, Shieh WJ, Paddock CD, Zaki SR
Jazyk: angličtina
Zdroj: Emerging infectious diseases [Emerg Infect Dis] 2013 Jun; Vol. 19 (6), pp. 886-91.
DOI: 10.3201/eid1906.130173
Abstrakt: During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.
Databáze: MEDLINE
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