Meta-omic characterization of prokaryotic gene clusters for natural product biosynthesis.

Autor: Schofield MM; Life Sciences Institute and Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Sherman DH
Jazyk: angličtina
Zdroj: Current opinion in biotechnology [Curr Opin Biotechnol] 2013 Dec; Vol. 24 (6), pp. 1151-8. Date of Electronic Publication: 2013 May 31.
DOI: 10.1016/j.copbio.2013.05.001
Abstrakt: Microorganisms produce a remarkable selection of bioactive small molecules. The study and exploitation of these secondary metabolites have traditionally been restricted to the cultivable minority of bacteria. Rapid advances in meta-omics challenge this paradigm. Breakthroughs in metagenomic library methodologies, direct sequencing, single cell genomics, and natural product-specific bioinformatic tools now facilitate the retrieval of previously inaccessible biosynthetic gene clusters. Similarly, metaproteomic developments enable the direct study of biosynthetic enzymes from complex microbial communities. Additional methods within and beyond meta-omics are also in development. This review discusses recent reports in these arenas and how they can be utilized to characterize natural product biosynthetic gene clusters and pathways.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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