Apoptotic induction of skin cancer cell death by plant extracts.
Autor: | Thuncharoen W; Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand., Chulasiri M, Nilwarangkoon S, Nakamura Y, Watanapokasin R |
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Jazyk: | angličtina |
Zdroj: | Journal of the Medical Association of Thailand = Chotmaihet thangphaet [J Med Assoc Thai] 2013 Jan; Vol. 96 Suppl 1, pp. S60-4. |
Abstrakt: | Objective: The aim of the present study was to investigate the effects of plant extracts on cancer apoptotic induction. Material and Method: Human epidermoid carcinoma A431 cell line, obtained from the American Type Culture Collection (ATCC, Manassas, VA), was maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 degrees C, 5% carbon dioxide (CO2). Plant extract solutions were obtained from S & J international enterprises public company limited. These plant extracts include 50% hydroglycol extracts from Etlingera elatior (Jack) R.M.Smith (torch ginger; EE), Rosa damascene (damask rose; DR) and Rafflesia kerrii Meijer (bua phut; RM). The cell viability, time and dose dependency were determined by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. A431 cells were treated with the plant extracts and stained with Hoechst 33342 fluorescent staining dye. Results: Cell viability was demonstrated by the inhibitory concentration 50% (IC50). The anti-proliferative effects were shown to be dependent on time and dose. Typical characteristics of apoptosis which are cell morphological changes and chromatin condensation were clearly observed. Conclusion: The plant extracts was shown to be effective for anti-proliferation and induction of apoptosis cell death in skin cancer cells. Therefore, mechanisms underlying the cell death and its potential use for treatment of skin cancer will be further studied. |
Databáze: | MEDLINE |
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