| Autor: |
Mamadou S; Laboratory of Bacteriology-Virology, Faculty of Health Sciences, Abdou Moumouni University, P.O. Box 237, Niamey, Niger ; National Reference Laboratory for STI/HIV/TB, P.O. Box 10 146, Niamey, Niger., Hanki Y, Ali Maazou AR, Aoula B, Diallo S |
| Jazyk: |
angličtina |
| Zdroj: |
ISRN microbiology [ISRN Microbiol] 2011 Nov 03; Vol. 2011, pp. 797463. Date of Electronic Publication: 2011 Nov 03 (Print Publication: 2011). |
| DOI: |
10.5402/2011/797463 |
| Abstrakt: |
The objective of the study was to estimate the prevalence of transmitted resistance to antiretroviral of HIV-1 circulating in Niger. We collected plasmas from 96 drug-naive patients followed up in the main HIV/AIDS Care Center of Niamey, the capital city of Niger. After RNA extraction and retrotranscription to proviral DNA, nested PCR was performed to amplify PR (codons 1-99) and RT (codons 1-240) fragments for sequencing. Sequences were analysed for phylogeny, then for resistance-associated mutations according to IAS-USA and Stanford's lists of mutations. We characterized six HIV-1 genetic variants: CRF02-AG (56.3%), CRF30_0206 (15.6%), subtype G (15.6%), CRF06_cpx (9.4%), CRF11_cpx (2.1%), and CRF01_AE (1%). About 8.3% of HIV strains had at least 1 resistance mutation: 4 strains with at least 1 mutation to NRTI, 5 for NNRTI, and 1 for PI, respectiveley 4.2%, 5.2%, and 1.0%. These preliminary results gave enough information for the need of instauring HIV drug resistance national surveillance. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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