Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells.

Autor: Khanna M; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202-5126, USA., Imasaki T, Chikwana VM, Perez-Miller S, Hunter GO, Mosley A, Takagi Y, Hurley TD
Jazyk: angličtina
Zdroj: Protein expression and purification [Protein Expr Purif] 2013 Aug; Vol. 90 (2), pp. 78-83. Date of Electronic Publication: 2013 May 24.
DOI: 10.1016/j.pep.2013.05.007
Abstrakt: We have successfully expressed and purified active human glycogen synthase-1 (hGYS1). Successful production of the recombinant hGYS1 protein was achieved by co-expression of hGYS1 and rabbit glycogenin (rGYG1) using the MultiBac baculovirus expression system (BEVS). Functional measurements of activity ratios of hGYS1 in the absence and presence of glucose-6-phosphate and treatment with phosphatase indicate that the expressed protein is heavily phosphorylated. We used mass spectrometry to further characterize the sites of phosphorylation, which include most of the known regulatory phosphorylation sites, as well as several sites unique to the insect cell over-expression. Obtaining large quantities of functional hGYS1 will be invaluable for future structural studies as well as detailed studies on the effects on specific sites of phosphorylation.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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