| Autor: |
Tan CS; Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. ctan@bidmc.harvard.edu, Broge TA Jr, Seung E, Vrbanac V, Viscidi R, Gordon J, Tager AM, Koralnik IJ |
| Jazyk: |
angličtina |
| Zdroj: |
PloS one [PLoS One] 2013 May 20; Vol. 8 (5), pp. e64313. Date of Electronic Publication: 2013 May 20 (Print Publication: 2013). |
| DOI: |
10.1371/journal.pone.0064313 |
| Abstrakt: |
Progressive Multifocal Leukoencephalopathy (PML) is an often fatal disease caused by the reactivation of the JC virus (JCV). Better understanding of viral-host interactions has been hampered by the lack of an animal model. Engrafting NOD/SCID/IL-2-Rg (null) mice with human lymphocytes and thymus, we generated a novel animal model for JCV infection. Mice were inoculated with either a PML isolate, JCV Mad-4, or with JCV CY, found in the kidney and urine of healthy individuals. While mice remained asymptomatic following inoculation, JCV DNA was occasionally detected in both the blood and the urine compartments. Mice generated both humoral and cellular immune responses against JCV. Expressions of immune exhaustion marker, PD-1, on lymphocytes were consistent with response to infection. Using this model we present the first in vivo demonstration of virological and immunological differences between JCV Mad-4 and CY. This model may prove valuable for studying JCV host immune responses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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