| Autor: |
Btesh J; Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom., Fischer MJM; Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom.; Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, 91052 Erlangen, Germany., Stott K; Department of Biochemistry, University of Cambridge, Cambridge CB2 1 GA, United Kingdom., McNaughton PA; Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom. |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2013 May 22; Vol. 33 (21), pp. 9184-9193. |
| DOI: |
10.1523/JNEUROSCI.4991-12.2013 |
| Abstrakt: |
Inflammation causes hyperalgesia, an enhanced sensitivity to noxious stimuli. Transient receptor potential vanilloid 1 (TRPV1), a thermo-TRP ion channel activated by painful levels of heat, is an important contributor because hyperalgesia is reduced when TRPV1 is either genetically deleted or pharmacologically blocked. Inflammatory mediators such as prostaglandin-E2 or bradykinin cause hyperalgesia by activating cellular kinases that phosphorylate TRPV1, a process that has recently been shown to rely on a scaffolding protein, AKAP79, to target the kinases to TRPV1. Here we use Förster resonance energy transfer, immunoprecipitation, and TRPV1 membrane trafficking experiments to identify a key region on AKAP79, between amino acids 326-336, which is responsible for its interaction with TRPV1. A peptide identical to this domain inhibited sensitization of TRPV1 in vitro, and when covalently linked to a TAT peptide to promote uptake across the cell membrane the peptide inhibited in vivo inflammatory hyperalgesia in mice. Critically, it did so without affecting pain thresholds in the absence of inflammation. These results suggest that antagonizing the TRPV1-AKAP79 interaction will be a useful strategy for inhibiting inflammatory hyperalgesia. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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