| Autor: |
Vázquez-Castilla S; Phytochemicals and Food Quality Group, Food Biotechnology Department, Instituto de la Grasa (CSIC), Seville, Spain., Jaramillo-Carmona S, Fuentes-Alventosa JM, Jiménez-Araujo A, Rodriguez-Arcos R, Cermeño-Sacristán P, Espejo-Calvo JA, Guillén-Bejarano R |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of agricultural and food chemistry [J Agric Food Chem] 2013 Jul 03; Vol. 61 (26), pp. 6250-8. Date of Electronic Publication: 2013 Jun 18. |
| DOI: |
10.1021/jf401462w |
| Abstrakt: |
The main goal of this study was the optimization of a HPLC-MS method for the qualitative and quantitative analysis of asparagus saponins. The method includes extraction with aqueous ethanol, cleanup by solid phase extraction, separation by reverse phase chromatography, electrospray ionization, and detection in a single quadrupole mass analyzer. The method was used for the comparison of selected genotypes of Huétor-Tájar asparagus landrace and selected varieties of commercial diploid hybrids of green asparagus. The results showed that while protodioscin was almost the only saponin detected in the commercial hybrids, eight different saponins were detected in the Huétor-Tájar asparagus genotypes. The mass spectra indicated that HT saponins are derived from a furostan type steroidal genin having a single bond between carbons 5 and 6 of the B ring. The total concentration of saponins was found to be higher in triguero asparagus than in commercial hybrids. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
