Replication of white spot syndrome virus (WSSV) in the polychaete Dendronereis spp.

Autor: Desrina; Aquaculture and Fisheries Group, Wageningen University, The Netherlands; Fisheries Department, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang, Indonesia. rinadesrina@yahoo.com, Verreth JA, Prayitno SB, Rombout JH, Vlak JM, Verdegem MC
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2013 Sep; Vol. 114 (1), pp. 7-10. Date of Electronic Publication: 2013 May 14.
DOI: 10.1016/j.jip.2013.05.002
Abstrakt: This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated in a naturally infected non-crustacean host.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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