Corneal cell response after flap creation using a mechanical microkeratome or a 200 kHz femtosecond laser.
| Autor: | Mayer WJ; Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. wolfgang.j.mayer@med.uni-muenchen.de, Grueterich M, Wolf AH, Lackerbauer CA, Eibl K, Kampik A, Kook D |
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| Jazyk: | angličtina |
| Zdroj: | Journal of cataract and refractive surgery [J Cataract Refract Surg] 2013 Jul; Vol. 39 (7), pp. 1088-92. Date of Electronic Publication: 2013 May 13. |
| DOI: | 10.1016/j.jcrs.2013.01.047 |
| Abstrakt: | Purpose: To compare the inflammatory cell response within the corneal flap interface created by a mechanical microkeratome and a femtosecond laser. Setting: Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. Design: Experimental in vitro study. Methods: Corneoscleral buttons of 12 enucleated human eyes not suitable for transplantation were put into organ culture. Corneal flaps were created using a 200 kHz femtosecond laser (Visumax) (femtosecond group) or a mechanical microkeratome (Amadeus) (microkeratome group). Flaps were not lifted after treatment. In 2 corneas, no treatment was performed (control group). Corneas were kept in organ culture for 12 hours thereafter. To evaluate cell-mediated immune reaction, immunofluorescent staining for leucocytes (cluster of differentiation 45) and specifically for dendritic cells (human leukocyte antigen-DR) was performed in every group. A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to determine apoptosis reaction. Results: The ratio of dendritic cells in the femtosecond group compared with the microkeratome group was 1.2 (P=.02), the ratio of leucocytes was 1.4 (P=.06), and the ratio of apoptotic cells was 1.0 (P=.59). There was no marked significant difference in the distribution of inflammatory cell reaction. The control group showed neither specific inflammatory reaction nor apoptosis. Conclusion: This in vitro series of human corneas showed similar inflammatory tissue reaction after femtosecond laser-assisted and microkeratome-assisted flap creation (P<.05). Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned. (Copyright © 2013 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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