| Autor: |
Wages SA; Control Technology Branch, Centers for Disease Control, Atlanta, GA 30333., Patchen LC, Churchill FC |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1990 Apr 27; Vol. 527 (1), pp. 115-26. |
| DOI: |
10.1016/s0378-4347(00)82088-5 |
| Abstrakt: |
We describe a high-performance liquid chromatographic method with electrochemical detection for quantifying pyronaridine in rhesus monkey (Macaca mulata) blood and urine samples. The detection limit is 20 ng/ml at a signal-to-noise ratio of 4 in 0.5-ml samples of blood or urine. Blood analysis includes a liquid-liquid extraction and a subsequent solid-phase extraction that removes an interferent present in blood. For urine, a back-extraction is substituted for the solid-phase extraction step. The method uses an analogue of amodiaquine as internal standard, a 10-microns rigid macroporous styrene-divinylbenzene copolymer column and a mobile phase of 1% (v/v) triethylamine in methanol-water (34:66, v/v). The method was applied to samples of blood and urine from a monkey after a single intramuscular dose of pyronaridine tetraphosphate (160 mg as base). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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