Modified silaffin R5 peptides enable encapsulation and release of cargo molecules from biomimetic silica particles.

Autor: Lechner CC; University of Vienna, Department of Chemistry, Institute of Biological Chemistry, Währinger Strasse 38, 1090 Vienna, Austria., Becker CF
Jazyk: angličtina
Zdroj: Bioorganic & medicinal chemistry [Bioorg Med Chem] 2013 Jun 15; Vol. 21 (12), pp. 3533-41. Date of Electronic Publication: 2013 Apr 15.
DOI: 10.1016/j.bmc.2013.04.006
Abstrakt: Biomimetic silica formation has attracted increasing interest over the last decade for numerous biotechnological applications due to the favorable mild reaction conditions. Inspired from silica biogenesis in diatoms, peptide variants derived from native silaffins have been used for silica formation in vitro. Here a generally applicable route for covalently linking a cargo molecule to the R5 silaffin peptide via a disulfide linkage is established. The peptide CG12AB, a peptide ligand of the epidermal growth factor receptor, was chosen as model. The ability of such silaffin-cargo conjugates to encapsulate the cargo molecule during silaffin-mediated silica precipitation is demonstrated. Cargo release from silica material under different conditions was analyzed. The results obtained here provide a rational basis for developing engineered R5 silaffin peptides into efficient tools for silica precipitation as well as for entrapment and release of cargo molecules under physiological conditions.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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