Heat shock induces interferon-TAU gene expression by in vitro-produced bovine blastocysts.

Autor: Hickman CF; SRUC, Edinburgh, UK., Clinton M, Ainslie A, Ashworth CJ, Rooke JA
Jazyk: angličtina
Zdroj: American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2013 Sep; Vol. 70 (3), pp. 177-81. Date of Electronic Publication: 2013 May 03.
DOI: 10.1111/aji.12131
Abstrakt: Problem: The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro.
Method of Study: In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified.
Results: Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not.
Conclusion: The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.
(© 2013 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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