Mesodermal and neural crest derived ovine tibial and mandibular osteoblasts display distinct molecular differences.

Autor: Reichert JC; Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Ave, 4059 Kelvin Grove, Queensland, Australia., Gohlke J, Friis TE, Quent VM, Hutmacher DW
Jazyk: angličtina
Zdroj: Gene [Gene] 2013 Aug 01; Vol. 525 (1), pp. 99-106. Date of Electronic Publication: 2013 Apr 28.
DOI: 10.1016/j.gene.2013.04.026
Abstrakt: Mandibular osteoblasts originate from the neural crest and deposit bone intramembranously, mesoderm derived tibial osteoblasts by endochondral mechanisms. Bone synthesized by both cell types is identical in structure, yet functional differences between the two cell types may exist. Thus, both matched juvenile and adult mandibular and tibial osteoblasts were studied regarding their proliferative capacity, their osteogenic potential and the expression of osteogenic and origin related marker genes. Juvenile tibial cells proliferated at the highest rate while juvenile mandibular cells exhibited higher ALP activity depositing more mineralized matrix. Expression of Hoxa4 in tibial cells verified their mesodermal origin, whereas very low levels in mandibular cells confirmed their ectodermal descent. Distinct differences in the expression pattern of bone development related genes (collagen type I, osteonectin, osteocalcin, Runx2, MSX1/2, TGF-β1, BAMBI, TWIST1, β-catenin) were found between the different cell types. The distinct dissimilarities in proliferation, alkaline phosphatase activity, the expression of characteristic genes, and mineralization may aid to explain the differences in bone healing time observed in mandibular bone when compared to long bones of the extremities.
(Copyright © 2013. Published by Elsevier B.V.)
Databáze: MEDLINE
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