Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence.

Autor: Reddington SC; School of Biosciences, Cardiff University, Cardiff, UK., Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2013 Jun 03; Vol. 52 (23), pp. 5974-7. Date of Electronic Publication: 2013 Apr 25.
DOI: 10.1002/anie.201301490
Abstrakt: Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.
Databáze: MEDLINE