Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity.

Autor: Yu F; Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. entyf@nus.edu.sg, Sim AC, Li C, Li Y, Zhao X, Wang DY, Loh KS
Jazyk: angličtina
Zdroj: The Laryngoscope [Laryngoscope] 2013 Aug; Vol. 123 (8), pp. 1903-11. Date of Electronic Publication: 2013 Apr 24.
DOI: 10.1002/lary.24003
Abstrakt: Objectives/hypothesis: Cancer stem cells have been reported as a new therapeutic target in many cancers, but their existence in nasopharyngeal carcinoma (NPC) is largely unknown. This study was conducted to determine cancer stem-like cells in NPC cell line.
Study Design: Basic science experimental study.
Methods: Aldehyde dehydrogenase (ALDH) activity, a putative functional marker for cancer stem cells, was assessed in Epstein-Barr virus-associated NPC cell line C666-1 cells. The ability of cells with high and low ALDH activity to proliferate, resist therapy, and initiate tumor formation was compared.
Results: Enrichment of cancer stem-like cells (with high ALDH activity) in C666-1 was associated with a significantly greater ability to proliferate, be clonogenic, resist chemotherapy drugs and radiation, reconstitute a heterogeneous population, and express pluripotent markers. Furthermore, subcutaneous injection of these cells into immunodeficient nude mice resulted in a tendency of tumor formation at a higher rate as compared to cells with low ALDH activity.
Conclusions: These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells.
(© 2013 The American Laryngological, Rhinological and Otological Society, Inc.)
Databáze: MEDLINE
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