Lipopolysaccharide (LPS) alters phosphatidylcholine metabolism in elicited peritoneal macrophages.
Autor: | Grove RI; Bristol-Myers Company, PRDD, Wallingford, Connecticut 06492., Allegretto NJ, Kiener PA, Warr GA |
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Jazyk: | angličtina |
Zdroj: | Journal of leukocyte biology [J Leukoc Biol] 1990 Jul; Vol. 48 (1), pp. 38-42. |
DOI: | 10.1002/jlb.48.1.38 |
Abstrakt: | We investigated the effects of LPS on mouse peritoneal macrophage phospholipids using radiolabeled precursors. LPS (200 ng/ml) stimulated incorporation of [32P] into all classes of phospholipids within 0.5 hr, and after 2 hr the increase was 60% greater than controls. Separation of the phospholipid classes by thin-layer chromatography revealed a selective increase in incorporation of label into phosphatidylcholine (PC) (90% increase compared to approximately 50% in the other phospholipids). In macrophages labeled with [3H]-choline, LPS stimulated both the incorporation of label into PC and the release of incorporated label into the medium. The time dependencies of stimulated [3H] release and [32P] incorporation were similar. These data are consistent with the hypothesis that LPS activates macrophages via a PC-specific phospholipase-dependent mechanism. |
Databáze: | MEDLINE |
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