| Autor: |
Kostina EV, Riabinin VA, Maksakova GA, Siniakov AN |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2012 Nov-Dec; Vol. 38 (6), pp. 676-82. |
| DOI: |
10.1134/s1068162012060052 |
| Abstrakt: |
The microchip for influenza A subtyping was developed, functioning on a principle "one spot--one subtype". Each spot contains the set of oligonucleotide probes, specific for a particular subtype of hemagglutinin, neuraminidase or matrix gene. Reliability of the proposed chip version is the same as for earlier created in our group full-size microchip for separate hemagglutinin and neuraminidase subtyping. To visualize the image, analyzed DNA can be labeled by either fluorescent dye or biotin with the further fixation in system streptavidin-gold nanoparticles and image development by silver precipitation. In the second case common version of scanner can be used for the image analysis, that essentially simplifies procedure of influenza A subtyping. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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