Analysis of phenolic compounds in Portuguese wild and commercial berries after multienzyme hydrolysis.

Autor: Pimpão RC; Instituto de Tecnologia Quı́mica e Biológica, Universidade Nova de Lisboa , Av. da República, EAN, 2781-901 Oeiras, Portugal., Dew T, Oliveira PB, Williamson G, Ferreira RB, Santos CN
Jazyk: angličtina
Zdroj: Journal of agricultural and food chemistry [J Agric Food Chem] 2013 May 01; Vol. 61 (17), pp. 4053-62. Date of Electronic Publication: 2013 Apr 22.
DOI: 10.1021/jf305498j
Abstrakt: Berry fruits are a good source of phenolic compounds and thus, potentially beneficial to health. Phenolic compounds are mainly present as a variety of conjugated forms, either with sugars via O-glycosidic bonds or with other polyols as esters. This chemodiversity makes characterization and identification highly demanding. Selected varieties of commercial blueberries, raspberries and blackberries and the two wild berries Portuguese crowberry and strawberry tree fruits were characterized for individual phenolic content by liquid chromatography-diode array detection and mass spectrometry (HPLC-DAD-MS) after hydrolysis by a novel combination of the fungal glycosidases hesperidinase and cellulase. This approach is shown to be a simple alternative to other existing methods for analysis of plant phenolic compound aglycones. The hydrolysis of glycosides and organic acid esters is efficient and less aggressive than acid and alkaline hydrolysis. This method is able to disclose new sources of dietary phenolic compounds, and the potential usefulness of Portuguese crowberry and strawberry tree fruit is herein demonstrated.
Databáze: MEDLINE