HIV-1 Vpu affects the anterograde transport and the glycosylation pattern of NTB-A.

Autor: Bolduan S; Institute of Virology, University of Erlangen-Nuremberg, Germany., Hubel P, Reif T, Lodermeyer V, Höhne K, Fritz JV, Sauter D, Kirchhoff F, Fackler OT, Schindler M, Schubert U
Jazyk: angličtina
Zdroj: Virology [Virology] 2013 Jun 05; Vol. 440 (2), pp. 190-203. Date of Electronic Publication: 2013 Mar 22.
DOI: 10.1016/j.virol.2013.02.021
Abstrakt: HIV-1 Vpu induces downregulation of cell surface NTB-A to evade lysis of HIV-1 infected cells by NK cells. Here we show that Vpu affects the anterograde transport and the glycosylation pattern of NTB-A by a mechanism that is distinct from the Vpu induced downregulation of CD4 and tetherin. In the presence of Vpu, only the high mannose form of NTB-A was detectable, suggesting that Vpu prevented the formation of the mature form of NTB-A. This phenomenon is associated with the ability of Vpu to downregulate cell surface NTB-A by retention of NTB-A within the Golgi-compartment. Furthermore, the Vpu-mediated effect on NTB-A glycosylation is highly conserved among Vpu proteins derived from HIV-1 and SIV and corresponds to the level of downregulation of NTB-A. Together, these results suggest that the reduction of NTB-A from the cell surface is associated with the Vpu-mediated effect on the glycosylation pattern of newly synthesized NTB-A molecules.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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