Activity of thymidilate "salvage pathway" enzymes in human gastric cancer and blood serum: correlation with treatment modalities.
Autor: | Borzenko BG; Department of Chemistry, Maksim Gorky National Medical University, Donetsk, Ukraine. borzenko.bg@rambler.ru, Bakurova EM, Popovich YA, Sidyuk EA, Popovich AY |
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Jazyk: | angličtina |
Zdroj: | Experimental oncology [Exp Oncol] 2013 Mar; Vol. 35 (1), pp. 37-40. |
Abstrakt: | Unlabelled: A comparative study of enzyme activity features of thymidilate "salvage pathway" synthesis in blood serum and tissues of different age patients with gastric cancer (T(3-4)N(0-x)M(0)) was carried out. Aim: To evaluate the diagnostic relevance of thymidilate metabolism enzymes activities and their association with tumor growth. Methods: Enzymes activities were determined by the radioisotope method and spectrophotometrically in tumor tissues and blood serum of 74 patients. Results: It was demonstrated that thymidine phosphorylase activity in gastric tumors is lower by 2.6 times as compared to non-neoplastic mucosa of resection margin. This being accompanied by decrease of its activity in the blood serum (from 47.9 ± 2.6 to 14.65 ± 2.4 nmol/min·mg, p < 0.001). An increase of thymidine kinase activity was revealed both in tumor tissues (more than 3.5 times) and in blood serum (from 3.9 ± 0,7 nmol/mg·h, to 6.8 ± 1.0 nmol/mg·h, p < 0.01). Changes in their activity in the postoperative period depended on the type of surgical procedure and tumor eradication. Conclusion: It could be suggested that control of individual dynamics of the enzymes activities in blood serum may be used as informative tool for monitoring of patients and treatment optimization. |
Databáze: | MEDLINE |
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