| Autor: |
Sun YY; Department of Hematology, Southeast University Medical School, Nanjing, Jiangsu Province, China., Wang SJ, Chen BA, Xia GH, Ding JH, Gao C, Song HH, Guo QL, Zhang HW, Li CR, Zhou JF |
| Jazyk: |
čínština |
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2013 Feb; Vol. 21 (1), pp. 105-9. |
| DOI: |
10.7534/j.issn.1009-2137.2013.01.022 |
| Abstrakt: |
The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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