| Autor: |
Straub WE; Life Technologies, San Francisco, CA, USA., Nishida CR, de Montellano PR |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 987, pp. 107-13. |
| DOI: |
10.1007/978-1-62703-321-3_9 |
| Abstrakt: |
Unlike many hemoproteins, the prosthetic heme group of most cytochrome P450 enzymes cannot be extracted and replaced by modified heme groups. Here, we describe a procedure for generating a cytochrome P450 enzyme (CYP119) with cobalt protoporphyrin IX as its prosthetic group. This is achieved by expressing the protein in Escherichia coli in iron-limited medium and adding cobalt to the medium at the moment that inducible protein expression is initiated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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