| Autor: |
Levadny V; Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan., Tsuboi TA, Belaya M, Yamazaki M |
| Jazyk: |
angličtina |
| Zdroj: |
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2013 Mar 26; Vol. 29 (12), pp. 3848-52. Date of Electronic Publication: 2013 Mar 12. |
| DOI: |
10.1021/la304662p |
| Abstrakt: |
We investigated the effects of tension induced by micropipet aspiration on giant unilamellar vesicles (GUVs) composed of dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylcholine (DOPC). We analyzed the time course of the fraction of intact GUVs among all of the GUVs under constant tension σ and obtained the rate constants of pore formation kp(σ). To determine kp, we developed an approach using the mean first passage time. The fitting of the theoretical curves of kp versus σ to the experimental data determined the line tension of a prepore, Γ. The value of Γ of a DOPG/DOPC bilayer was smaller than that of a DOPC bilayer. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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