Reversal of creatine kinase translational repression by 3' untranslated sequences.

Autor: Ch'ng JL; Department of Medicine, Duke University, Durham, NC 27710., Shoemaker DL, Schimmel P, Holmes EW
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 1990 May 25; Vol. 248 (4958), pp. 1003-6.
DOI: 10.1126/science.2343304
Abstrakt: A subline of U937 cells (U937D) was obtained in which creatine kinase B (CK-B) messenger RNA was present and bound to ribosomes, but CK activity was undetectable. Transformation of U937D cells with retrovirus vectors that contain the 3' untranslated region (3' UTR) of CK-B messenger RNA exhibited CK activity with no change in abundance of CK-B mRNA. The 3' UTR formed a complex in vitro with a component of S100 extracts from wild-type cells. This binding activity was not detectable in S100 extracts from cells that expressed CK activity after transformation with the 3' UTR-containing vector. These results suggest that translation of CK-B is repressed by binding of a soluble factor or factors to the 3' UTR.
Databáze: MEDLINE