| Autor: |
Tarburton JP; Department of Nuclear Medicine, Veterans Administration Medical Center, San Diego, CA 92161., Halpern SE, Hagan PL, Sudora E, Chen A, Fridman DM, Pfaff AE |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biological response modifiers [J Biol Response Mod] 1990 Apr; Vol. 9 (2), pp. 221-30. |
| Abstrakt: |
Studies were performed to determine in vitro and in vivo effects of acetylation on Fab' fragments of ZCE-025, a monoclonal anti-CEA antibody. Isoelectric focusing revealed a drop in isoelectric point of 1.7 pI units following acetylation. Biodistribution studies of acetylated and nonacetylated [111In]Fab' were performed in normal BALB/c mice and in nude mice bearing the T-380 CEA-producing human colon tumor. The acetylated fragments remained in the vascular compartment longer and had significantly diminished renal uptake of 111In compared to controls. While acetylation itself effected a 50% drop in immunoreactivity, tumor uptake of the acetylated and nonacetylated 111In-labeled Fab' fragments was comparable, with the exception of one data point, through 72 h. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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