Expression and characterization of the nitrile reductase queF from E. coli.
| Autor: | Moeller K; Biokatalyse & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands., Nguyen GS, Hollmann F, Hanefeld U |
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| Jazyk: | angličtina |
| Zdroj: | Enzyme and microbial technology [Enzyme Microb Technol] 2013 Mar 05; Vol. 52 (3), pp. 129-33. Date of Electronic Publication: 2012 Dec 14. |
| DOI: | 10.1016/j.enzmictec.2012.12.003 |
| Abstrakt: | The expression and characterization of a nitrile reductase from Escherichia coli K-12 (EcoNR), a newly discovered enzyme class, is described. This enzyme has a potential application for an alternative nitrile reduction pathway. The enzyme activity towards its natural substrate, preQ(0), was demonstrated and optimal working conditions were found to be at 37°C and at pH 7 with Tris buffer. (Copyright © 2012 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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