| Autor: |
Dreifuss AA; Pharmacology Department, Federal University of Paraná, Brazil., Bastos-Pereira AL, Fabossi IA, Lívero FA, Stolf AM, Alves de Souza CE, Gomes Lde O, Constantin RP, Furman AE, Strapasson RL, Teixeira S, Zampronio AR, Muscará MN, Stefanello ME, Acco A |
| Jazyk: |
angličtina |
| Zdroj: |
PloS one [PLoS One] 2013; Vol. 8 (2), pp. e54618. Date of Electronic Publication: 2013 Feb 07. |
| DOI: |
10.1371/journal.pone.0054618 |
| Abstrakt: |
This study aimed to compare the anti-neoplastic effects of an Uncaria tomentosa (UT) brute hydroethanolic (BHE) extract with those of two fractions derived from it. These fractions are choroformic (CHCl3) and n-butanolic (BuOH), rich in pentacyclic oxindole alkaloids (POA) and antioxidant substances, respectively. The cancer model was the subcutaneous inoculation of Walker-256 tumour cells in the pelvic limb of male Wistar rat. Subsequently to the inoculation, gavage with BHE extract (50 mg.kg(-1)) or its fractions (as per the yield of the fractioning process) or vehicle (Control) was performed during 14 days. Baseline values, corresponding to individuals without tumour or treatment with UT, were also included. After treatment, tumour volume and mass, plasma biochemistry, oxidative stress in liver and tumour, TNF-α level in liver and tumour homogenates, and survival rates were analysed. Both the BHE extract and its BuOH fraction successfully reduced tumour weight and volume, and modulated anti-oxidant systems. The hepatic TNF-α level indicated a greater effect from the BHE extract as compared to its BuOH fraction. Importantly, both the BHE extract and its BuOH fraction increased the survival time of the tumour-bearing animals. Inversely, the CHCl3 fraction was ineffective. These data represent an in vivo demonstration of the importance of the modulation of oxidative stress as part of the anti-neoplastic activity of UT, as well as constitute evidence of the lack of activity of isolated POAs in the primary tumour of this tumour lineage. These effects are possibly resulting from a synergic combination of substances, most of them with antioxidant properties. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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