MRI analysis of cerebellar and vestibular developmental phenotypes in Gbx2 conditional knockout mice.

Autor: Szulc KU; Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA; Biomedical Imaging Program, New York University School of Medicine, New York, New York, USA., Nieman BJ, Houston EJ, Bartelle BB, Lerch JP, Joyner AL, Turnbull DH
Jazyk: angličtina
Zdroj: Magnetic resonance in medicine [Magn Reson Med] 2013 Dec; Vol. 70 (6), pp. 1707-17. Date of Electronic Publication: 2013 Feb 07.
DOI: 10.1002/mrm.24597
Abstrakt: Purpose: Our aim in this study was to apply three-dimensional MRI methods to analyze early postnatal morphological phenotypes in a Gbx2 conditional knockout (Gbx2-CKO) mouse that has variable midline deletions in the central cerebellum, reminiscent of many human cerebellar hypoplasia syndromes.
Methods: In vivo three-dimensional manganese-enhanced MRI at 100-µm isotropic resolution was used to visualize mouse brains between postnatal days 3 and 11, when cerebellum morphology undergoes dramatic changes. Deformation-based morphometry and volumetric analysis of manganese-enhanced MRI images were used to, respectively, detect and quantify morphological phenotypes in Gbx2-CKO mice. Ex vivo micro-MRI was performed after perfusion-fixation with supplemented gadolinium for higher resolution (50-µm) analysis.
Results: In vivo manganese-enhanced MRI and deformation-based morphometry correctly identified known cerebellar defects in Gbx2-CKO mice, and novel phenotypes were discovered in the deep cerebellar nuclei and the vestibulo-cerebellum, both validated using histology. Ex vivo micro-MRI revealed subtle phenotypes in both the vestibulo-cerebellum and the vestibulo-cochlear organ, providing an interesting example of complementary phenotypes in a sensory organ and its associated brain region.
Conclusion: These results show the potential of three-dimensional MRI for detecting and analyzing developmental defects in mouse models of neurodevelopmental diseases.
(Copyright © 2013 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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