Antiplasmodial potential of selected medicinal plants from eastern Ghats of South India.

Autor: Kaushik NK; Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India., Bagavan A, Rahuman AA, Mohanakrishnan D, Kamaraj C, Elango G, Zahir AA, Sahal D
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2013 May; Vol. 134 (1), pp. 26-32. Date of Electronic Publication: 2013 Feb 09.
DOI: 10.1016/j.exppara.2013.01.021
Abstrakt: Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitive (3D7) and (CQ)-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Studied extracts showed a spectrum of antiplasmodial activities ranging from (a) very good (IC(50)<10-10 μg/mL: Cyperus rotundus and Zingiber officinale); (b) good (IC(50), >10-15 μg/mL: Ficus religiosa and Murraya koenigii); (c) moderate (IC(50)>15-25 μg/mL: Ficus benghalensis); (d) poor activity (IC(50)>25-60 μg/mL) and (e) inactive (IC(50)>60 μg/mL). Resistance indices ranging from 0.78 to 1.28 suggest that some of these extracts had equal promise against the CQ resistant INDO strain of P. falciparum. Cytotoxicity assessment of the extracts against HeLa cell line using MTT assay revealed that the selectivity indices in the range of 3-15 suggesting a good margin of safety.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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