EDF-1 downregulates the CaM/Cn/NFAT signaling pathway during adipogenesis.

Autor: López-Victorio CJ; Department of Cell Biology, Center for Research and Advanced Studies-IPN, Apdo. Postal 14-740, México City 07000, Mexico., Velez-delValle C, Beltrán-Langarica A, Kuri-Harcuch W
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Mar 01; Vol. 432 (1), pp. 146-51. Date of Electronic Publication: 2013 Feb 01.
DOI: 10.1016/j.bbrc.2013.01.069
Abstrakt: The endothelial differentiation factor-1 (EDF-1) is a calmodulin binding protein that regulates calmodulin-dependent enzymes. In endothelial cells, this factor can form a protein complex with calmodulin. We analyzed the relationship between this factor and the members of calmodulin/calcineurin/nuclear factor of activated T-cells (NFAT) signaling pathway during adipogenesis of 3T3-F442A cells. We found that the expression of edf1 is upregulated during early adipogenesis, whereas that of calcineurin gene is lowered, suggesting that this pathway should be downregulated to allow for adipogenesis to occur. We also found that EDF-1 associates with calmodulin and calcineurin, most likely inactivating calcineurin. Our results showed that EDF-1 inactivates the calmodulin/calcineurin/NFAT pathway via sequestration of calmodulin, during early adipogenesis, and we propose a mechanism that negatively regulates the activation of calcineurin through a complex formation between EDF-1 and calmodulin. This finding raises the possibility that modulating this pathway might offer some alternatives to regulate adipose biology.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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