| Autor: |
Shi MM; Department of Pathology, The First Affiliated Hospital of China Medical University, Shenyang, China. shimumu2009@139.com, Xiong YL, Jia XS, Li X, Zhang L, Li XL, Wang EH |
| Jazyk: |
angličtina |
| Zdroj: |
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2013 Jun; Vol. 34 (3), pp. 1503-10. Date of Electronic Publication: 2013 Jan 29. |
| DOI: |
10.1007/s13277-013-0675-5 |
| Abstrakt: |
Most adult stem cells are in the G0 or quiescent phase of the cell cycle and account for only a small percentage of the cells in the tissue. Thus, isolation of stem cells from tissues for further study represents a major challenge. This study sought to enrich cancer stem cells and explore cancer stem-like cell clones using 5-fluorouracil (5-FU) in the lung adenocarcinoma cell line, SPC. Proliferation inhibition was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, according to which half maximal inhibitory concentration values were calculated. Expression levels of stem cell markers after treatment with 5-FU were examined using immunofluorescence and Western blotting. Additionally, side population (SP) cells were sorted using FACS. Properties of SP cells were evaluated by using Transwell, colony-forming assays, and tumor formation experiments. 5-FU greatly inhibits proliferation, especially of cells in S phase. SP cells possess greater invasive potential, higher clone-forming potential, and greater tumor-forming ability than non-SP cells. Treatment with 5-FU enriches the SP cells with stem cell properties in human lung adenocarcinoma cell lines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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