| Autor: |
Slompo C; Department of Biological Sciences, Bauru Dental School, USP - University of São Paulo, Bauru, SP, Brazil., Buzalaf CP, Damante CA, Martins GM, Hannas AR, Buzalaf MA, Oliveira RC |
| Jazyk: |
angličtina |
| Zdroj: |
Brazilian dental journal [Braz Dent J] 2012; Vol. 23 (6), pp. 629-34. |
| DOI: |
10.1590/s0103-64402012000600002 |
| Abstrakt: |
This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10(-6) M, 10(-5) M, 10(-4) M and 10(-3) M) for 24, 48, 72 and 96 h at 37ºC, 5% CO(2). Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10(-3) M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
